Compilation Pharm. Analysis II

MID SEM 2004/2005
PART 1
Answer two (2) out of the following:
(Marks = 5% per question)
1. “Instrumental analysis has given the scientist a new avenue of analysis.” Justify the above statement based on the advantages of modern instrumental analysis over conventional methods.

2. Write a short note on:
a. Shot noise
OR
b. Input transducers

3. Describe briefly the mechanism of inter system crossing.


PART 2
Answer one (1) out of the following:
(Marks = 10%)
1. Give a brief account of the different techniques involved for the noise reduction in instrumental analysis.

2. Discuss the detail mechanism of fluorescence with schematic diagram.


END SEM 2004/2005
PART 1
Answer three (3) out of the following:
(Marks = 5% per question)
1. Explain the deviation of Beer’s Law for polychromatic light.

2. What are the basic differences between normal phase and reverse phase HPLC column? Give examples of their column packing material.

3. Write short note on:
a. Pneumatic nebulizer
OR
b. Bathochromic shift

4. Describe the principle of a gas chromatographic instrumentation using a flow chart.


PART 2
Answer two (2) out of the following:
(Marks = 10% per question)
1. Discuss the working of the double beam (in time and in space) UV-vis spectrophotometer with the help of schematic diagram(s).

2. Describe how ionization takes place in inductively coupled plasma (ICP) in atomic emission spectroscopy. Explain with the help of schematic diagram(s).

3. Describe some commonly used detectors in liquid chromatography and their basis of operation.

END SEM 2007/2008
PART 1
Answer three (3) out of the following:
(Marks = 5% per question)
1. Briefly discuss the advantages of using micro bore column in HPLC analysis.

2. Explain the deviation of Beer’s Law due to polychromatic light.

3. Discuss the λmax shift caused by the change of polarity of the solvent in UV-vis spectrometry due to the presence of “n” electron in the molecule.

4. Describe the working of solvent delivery system (reciprocating pump).
OR
Sample introduction system (injector) used in modern HPLC system.


PART 2
Answer two (2) out of the following:
(Marks = 10% per question)
1. Describe in detail the working of inductively coupled plasma used as an excitation source in atomic emission spectroscopy with a schematic diagram.

2. Describe the working of double beam and single beam spectrophotometer in UV-vis spectrometry with a schematic diagram and highlight the advantages of the double beam instrument over single beam.

3. Name the commonly used detectors in Gas Chromatography (GC) and describe the basis of operations of any three (3) of them.